ALBA
Fluorescence Correlation Spectrometer
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ALBA
Fluorescence Correlation Spectrometer
ALBA is a dual channel spectrometer
for fluorescence correlation (or, fluctuation)
measurements optimized for either single-photon
or multi-photon excitation through an
epifluorescence microscope¡¡ |
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Application
1. Molecular Dynamics
2. Kinetic rate constants
3. Antibody-antigen interactions
4. Ligand-receptor interactions
5. Nucleic acid hybridization
6. Molecular aggregation, polydispersity,
and molecular weight
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ALBA
Fluorescence
Correlation Spectrometer
ALBA, spectroscopy
on single molecules
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| ACQUISITION
MODES |
ALBA
acquires either in time-mode
(the classical way: photons
acquired in time intervals
are counted) or in photon
mode (time delay between
one photon and the next
one is measured and an histogram
is built). A 2-channel data
acquisition card receives
the data stream from the
detectors and passes them
on to the computer. Raw
data are stored in a data
file for further processing.
No need anymore to reject
an autocorrelation function
because of large particles
interference. Raw data can
be purged from large particles
presence and the autocorrelation
function can be recalculated.
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| EXCITATION
MODALITY |
Single
photon excitation (using
an argon ion, kripton ion,
or dye- laser); or multi-
photon excitation (using
a Ti:Sapphire laser). |
| MICROSCOPES
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ALBA
can be interfaced to either
an Olympus or a Nikon epifluorescence
microscope. |
| DETECTORS
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Light
detectors are cooled photomultiplier
tubes or avalanche photodiodes.
The system is provided for
single channel or dual channel
data acquisition for cross-correlation
measurements. |
| THE
UNIQUE INSTRUMENT |
ALBA
can be upgraded for steady-state
and lifetime measurements.
All in a single instrument!
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